by Washington University in St. Louis

Overview of experiments and analysis. (a) Brugia malayi experiments. Adult worms collected from an experimental host (gerbils) and microfilariae of a known pedigree were used for methodology development. (b) Wuchereria bancrofti clinical isolates. Our genomics-based approach for analyzing worm burden was applied to samples obtained from human infections in Côte d’Ivoire, which were also utilized to develop probes for exome sequencing.

Researchers at Washington University School of Medicine in St. Louis have developed a new genomic-based approach that could aid global efforts to eliminate lymphatic filariasis, a parasitic roundworm infection spread by mosquitos. Also known as elephantiasis, the disease can be painful and disfiguring, with damage to the lymphatic system causing limb swelling, skin thickening and swelling of the scrotum.

The research is published in the journal eBioMedicine.

Co-led by Makedonka Mitreva, the Robert E. and Louise F. Dunn Professor of Medical Sciences, and Peter Fischer, a professor of medicine, the study describes a genomic-driven approach that distinguishes between the reinfection of an individual after successful treatment and the reemergence of an infection in an individual who was not fully cleared by treatment.

In 2022, about 325 million people received treatment for lymphatic filariasis as part of the World Health Organization's mass drug administration program to eliminate the parasite. This approach can be used for genomic surveillance, providing essential information for monitoring whether ongoing transmission is due to treatment failure or to human or mosquito migration.

More information: Young-Jun Choi et al, Distinguishing recrudescence from reinfection in lymphatic filariasis, eBioMedicine (2024). DOI: 10.1016/j.ebiom.2024.105188

Journal information: EBioMedicine 

Provided by Washington University in St. Louis