1.M6A-related bioinformatics analysis indicates that LRPPRC is an immune marker for ischemic stroke

DOI: 10.1038/s41598-024-57507-y

https://www.nature.com/articles/s41598-024-57507-y

This study investigates the role of 6-methyladenosine (m6A) modification in ischemic stroke (IS) pathogenesis. Using bioinformatics analysis of the GSE58294 dataset, seven differentially expressed genes (METTL3, WTAP, YWHAG, TRA2A, YTHDF3, LRPPRC, and HNRNPA2B1) were identified as m6A key regulatory genes. Clinical prediction models based on these genes were constructed and validated. IS patients were stratified based on these genes, revealing LRPPRC as an immune biomarker for IS. LRPPRC was associated with various cellular functions and correlated with immune proteins, suggesting its potential as a therapeutic target. These findings underscore LRPPRC's significance in IS immune microenvironment and suggest further research to elucidate its role in IS pathology and potential drug therapy.

2.Post-stroke arrhythmia could be a potential predictor for post-stroke depression

DOI: 10.1038/s41598-024-59789-8

https://www.nature.com/articles/s41598-024-59789-8

This study explores the relationship between post-stroke arrhythmia and post-stroke depression (PSD), hypothesizing that arrhythmia could serve as an early marker for PSD. Patients with first-ever ischemic stroke were enrolled, and the presence and severity of arrhythmia and PSD were assessed. Results revealed a higher prevalence of post-stroke arrhythmia, especially newly-detected, symptomatic, and poorly controlled arrhythmias, in patients with PSD. Additionally, patients with arrhythmia in the PSD group exhibited higher depression scores. Logistic regression identified newly-detected, symptomatic, and poorly controlled arrhythmias as independent predictors of PSD. The study suggests that post-stroke arrhythmia, particularly newly-detected and symptomatic cases, may indicate the presence and severity of PSD, highlighting the importance of arrhythmia management in reducing PSD risk and severity.

3.Integrating single-cell and spatially resolved transcriptomic strategies to survey the astrocyte response to stroke in male mice

DOI: 10.1038/s41467-024-45821-y

https://www.nature.com/articles/s41467-024-45821-y

This study introduces tDISCO, a platform for spatially resolved, single-cell transcriptomics and proteomics, to investigate the heterogeneity of astrocyte responses to cortical ischemic stroke in male mice. By integrating tDISCO with high-throughput spatial (Visium) and single-cell transcriptomics (10X Chromium) platforms, the study identifies two distinct astrocyte populations - proximal and distal to the injury site. tDISCO further delineates spatial boundaries and molecular profiles defining these populations. Proximal astrocytes exhibit differences in lipid shuttling, particularly enriched expression of Apoe and Fabp5. These findings provide insights into astrocyte roles in stroke and demonstrate the utility of tDISCO for spatially resolved single-cell experiments, offering a valuable resource for understanding astrocyte heterogeneity in neurological conditions like stroke.

4.Analysis of factors associated with depressive symptoms in stroke patients based on a national cross-sectional study

DOI: 10.1038/s41598-024-59837-3

https://www.nature.com/articles/s41598-024-59837-3

This study examines depressive symptoms prevalence and associated factors in Chinese stroke patients using data from the China Health and Retirement Longitudinal Study 2018. Of 963 participants, 57.8% exhibited depressive symptoms. Female sex, lower education level, dissatisfaction with spouse, life, and health, pain, and abnormal sleep were significantly associated with depressive symptoms. These findings highlight the importance of regular depression screening post-stroke. Implementing a supportive environment, effective health management, and lifestyle changes may enhance the mental well-being of stroke survivors.

5.Profiling of microglia nodules in multiple sclerosis reveals propensity for lesion formation

DOI: 10.1038/s41467-024-46068-3

https://www.nature.com/articles/s41467-024-46068-3

In this post-mortem study, microglia nodules (HLA-DR+ cell clusters) were examined to determine their association with multiple sclerosis (MS) lesion formation. The findings reveal that microglia nodules are linked to more severe MS pathology compared to stroke. Enhanced expression of genes associated with MS lesions, lipid metabolism, immune cell presence, cytokine production, and complement cascade activation was observed in MS microglia nodules. Additionally, these nodules showed increased phagocytosis of oxidized phospholipids and exhibited a distinct mitochondrial network. Notably, some MS microglia nodules encapsulated partially demyelinated axons. The study suggests that cytokine and immunoglobulin activation, coupled with oxidized phospholipid phagocytosis, may drive a microglia phenotype predisposed to MS lesion development.