by Universitat Pompeu Fabra - Barcelona

Close-up of insulinoma cells expressing insulin (red). Credit: Pompeu Fabra University (UPF)

A study led by Pompeu Fabra University describes the mechanism whereby insulinomas, a rare type of neuroendocrine tumor that affects pancreatic beta cells, form. According to the study, insulinomas are the result of the accumulation of rare mutations that lead to a homogeneous change in the epigenetic profile of pancreatic beta cells. This profile change causes beta cells to express unusually high levels of oncogenes, growth and transcription factors, and genes related to insulin production.

Insulinomas are rare pancreatic neuroendocrine tumors that involve the excessive growth of beta cells, which are responsible for secreting insulin. Often, they are diagnosed because they involve excessive insulin production that usually leads to hypoglycemia.

Each year, four people in one million are diagnosed with insulinomas, and in most cases, they are benign. If detected early and surgically removed, they have a good prognosis and only about in one in ten insulinomas are malignant.

In a study published in the journal Cell Genomics, a mechanism whereby beta cells undergo a transformation towards a neoplastic phenotype is proposed for the first time. "This is the accumulation of rare mutations that converge in a change in the epigenetic profile of beta cells," explains Mireia Ramos, co-first author of the study.

These mutations vary among the 42 insulinomas analyzed, and most accumulate in regulatory regions of the genome. "The uniqueness of insulinomas is that all of them, regardless of the mutations they have, end up acquiring the same epigenetic profile," adds Lorenzo Pasquali, director of the UPF Endocrine Regulatory Genomics Group, who led the research.

This new epigenetic profile causes tumor beta cells to lose their repression marks and, unlike healthy beta cells, have a series of active oncogenes, growth and transcription factors and genes related to insulin production, which alter their function.

Fragment of one of the figures of the paper. Simplified model illustrating the molecular mechanisms underlying insulinoma neoplastic transformation. Credit: Cell Genomics

The biology of beta cells

Apart from insulinomas, pancreatic beta cells are also implicated in other disabling diseases such as diabetes mellitus. Hence, "we are especially interested in understanding how these cells lose control, upset the expression of the genes that made them function normally and end up altering insulin secretion," Pasquali said.

Now, the group is already working to better understand the mechanism whereby excess beta cell growth occurs, which could, in the future, have therapeutic implications in the treatment of other diseases in which beta cells are altered.

This study also involved the Scientific Institute of Hospital San Raffaele in Milan (Italy); the University of Barcelona; IDIBELL, Bellvitge Biomedical Research Institute; the University of Colorado Boulder (U.S.); the Parc Taulí Research and Innovation Institute, I3PT; IRB Barcelona and the Diabetes and Associated Metabolic Diseases Networking Biomedical Research Center (CIBERDEM).

More information: Implications of noncoding regulatory functions in the development of insulinomas, Cell Genomics (2024). DOI: 10.1016/j.xgen.2024.100604. www.cell.com/cell-genomics/ful … 2666-979X(24)00198-8

Journal information: Cell Genomics 

Provided by Universitat Pompeu Fabra - Barcelona