by eLife

Tumor-reactive T cells induce transient cell-in-cell structure. (A) Mean percentage of cell-in-cell tumor formations following incubation of B16F10 cells with immune cells (n=4). (B) Representative 3D projection and horizontal sections (Z-stack) of B16F10 cells incubated with gp100-reactive CD8+ T cells. (C) Mean percentage of cell-in-cell tumor formations and single cells in tumor-bearing NSG-/- mice treated with DC adjuvant and anti-TRP1 antibodies (n=4). (D) Mean counts of cell-in-cell tumor formations in mouse cancer cell lines cultured overnight with activated CD8+ T cells (n=4). (E) Mean counts of cell-in-cell tumor formations in human cancer cell lines cultured overnight with activated allogeneic CD8+ T cells (n=4). (F–G) Representative images of freshly-isolated human melanoma cells incubated overnight with autologous tumor-reactive T cells. (H) Mean percentage of cell-in-cell tumor formations following incubation of tumor cells isolated from melanoma patient with autologous tumor-reactive T cells (four technical replicates). (I) Histological section of human melanoma in NSG-/- mice 7 days after treatment with patient autologous TIL and high-dose IL-2. (J) H&E staining of histological sections of involved lymph node from metastatic melanoma patient. (K) Immunostaining of involved lymph nodes from metastatic melanoma patient. (L) H&E staining of histological sections of primary and sentinel lymph nodes from untreated stage IV melanoma patients. (M) H&E staining of histological sections of untreated primary and relapsed melanoma patient. Credit: eLife (2022). DOI: 10.7554/eLife.80315

Scientists have shown how tumor cells evade immunotherapy by generating unique, temporary cell-in-cell structures, where the inner cells remain in tact and can return to single tumor cells.

These findings, published today in eLife, provide a novel theory as to how tumor cells avoid destruction by the immune system. They could also inform the development of treatments that combine immunotherapy with the timed inhibition of relevant signaling pathways in tumor cells.

"Cancer immunotherapy harnesses the body's immune system to fight cancer. Despite its remarkable success, the majority of patients who receive immunotherapy will only see their tumors shrink in size temporarily before returning, and these relapsed tumors will likely be resistant to immunotherapy treatment," says first author Amit Gutwillig, who was a Ph.D. student at the Carmi Lab, Tel Aviv University, Israel, at the time the study was carried out, and is now a Senior Researcher at Nucleai, Tel Aviv.

To identify how tumors relapse after immunotherapy, Carmi and the team began by comparing the genetic sequences of whole genomes in primary and relapsed tumors in the same patient. Their analysis suggested that relapsed tumors do not change dramatically following immunotherapy.

Next, the team studied this process in breast cancer and melanoma, using mouse models in which immunotherapy-resistant tumors had relapsed. They administered the mice with cells from treated tumors and allowed these cells to reach a palpable size. The team found that the cells were equally susceptible to the same immunotherapy approach as the parent tumor, although they relapsed sooner.

To better characterize the tumor cells that survived in mice following immunotherapy, the researchers isolated and studied the live tumor cells. They found that most of the cells responded to the presence of T cells—a type of immune cell that targets foreign particles—by organizing into temporary formations. These were made up of clusters of several tumor cell nuclei, which are surrounded by a single, multilayered membrane and a meshwork of cortical actin filaments. The inner cell of the formation was dense and appeared to be compacted within another cell.

To show that this result was not due to the isolation of the melanoma cells, the team also analyzed tumors with fluorescently labeled cell nuclei and membranes. They found that the cell-in-cell formation was more prevalent in immunotherapy-treated tumors, particularly in sites associated with tumor cell death. Further analysis indicated that roughly half of the tumor cells that survived immunotherapy were arranged in the cell-in-cell formation. Over time, these cells returned to a single-cell state, with similar structural features to those of the parental cell line.

The team next tested whether this phenomenon occurs in human cancers. To do this, they incubated tumor cell lines with pre-activated T cells from healthy donors. They discovered that the vast majority of breast, colon and melanoma tumor cells that survived T-cell killing organized into the cell-in-cell structure. A three-day observation of T cells interacting with tumor cells showed that these structures were dynamic, with individual tumor cells constantly forming and disseminating from the structure.

Finally, they tested the clinical relevance of this discovery by analyzing cancerous tissues from multiple organs of four stage 4 melanoma patients. These patients were undergoing surgical removal of primary and metastatic lymph nodes—that is, lymph nodes that had spread from the primary tumor. The researchers found that in all four patients, the cell-in-cell formation was highly abundant in the T-cell zone of the draining lymph nodes, but not in the primary tumors. Furthermore, in a patient with untreated recurrent melanoma, most of the cells in the primary tumor were single cells, whereas the recurrent tumors had an abundance of the cell-in-cell formations.

"This previously unknown mechanism of tumor resistance highlights a current limitation of immunotherapy," says senior author Yaron Carmi, Principal Investigator at the Department of Pathology, Sackler School of Medicine, Tel Aviv University. "Over the past decade, many clinical studies have used immunotherapy followed by chemotherapy. But our findings suggest that timed inhibition of relevant signaling pathways needs to occur alongside immunotherapy to prevent the tumor becoming resistant to subsequent treatments."

More information: Amit Gutwillig et al, Transient cell-in-cell formation underlies tumor relapse and resistance to immunotherapy, eLife (2022). DOI: 10.7554/eLife.80315

Journal information: eLife 

Provided by eLife