By:
Louis A. Cona, MD
Reviewed:
Robert J. Hancock
Unlock the potential of adult stem cells with our comprehensive guide. Delve into their biology, role in regenerative medicine, and future research prospects. Click to learn more!
Adult stem cells represent a cornerstone in the realm of regenerative medicine, offering transformative potential for treating various diseases and repairing tissues.
This article explores the diverse types and applications of stem cells, from their initial use in bone marrow transplants to the latest advancements in pluripotent and adult stem cell research.
What are Adult Stem Cells?
Adult stem cells, also known as somatic stem cells, are undifferentiated cells that exist throughout the body after development. Adult stem cells are found in all vascularized organs or tissues in adults.
The key function of adult stem cells is their ability to self-renew and differentiate into at least one specialized cell type (adult stem cell repair), allowing them to replace cells lost through normal wear and tear, injury, or disease.
Stem Cell Repair Mechanisms:
Adult stem cells contribute significantly to the maintenance and repair of body tissues.
They replace cells lost through normal processes, injury, or disease, thanks to their self-renewal and differentiation abilities.
Essential for organ homeostasis.
Critical in responding effectively to injuries.
Their migration to injury sites is a key aspect of their functionality.
Adult stem cells are now known to exist in various tissues, including the umbilical cord blood, bone marrow, adipose tissue, placenta, amniotic fluid, and menstrual blood.
Figure 1 - Adult Stem Cells Dyed
Where are Adult Stem Cells Found?
Adult stem cells can be found from various sources including:
Bone marrow: A rich source of Mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs). MSCs can differentiate into diverse cell types, while HSCs give rise to all blood cell types.
Adipose tissue: Adipose-derived stem cells (ADSCs) found in fat tissue are capable of differentiating into cell types like adipocytes, cartilage cells, and bone cells.
Umbilical cord blood: Contains MSCs, which are used in various therapeutic applications.
Placenta and amniotic fluid: These tissues are sources of MSCs used in regenerative medicine and tissue engineering.
Menstrual blood: Another source of MSCs, with potential therapeutic applications.
Nervous system: Neural stem cells (NSCs) located in specific brain areas can generate nerve cells, astrocytes, and oligodendrocytes.
Nucleus pulposus: These cells, found in spinal discs, have the potential to differentiate into disc tissue cells.
Salivary glands: Salivary gland stem cells are being explored for treating salivary gland dysfunction.
These diverse sources underscore the versatility of adult stem cells in medical research and therapy.
Types of Adult Stem Cells
Adult stem cells can be classified into several types based on their origin and differentiation potential:
Mesenchymal Stem Cells (MSCs): Initially isolated from bone marrow, these cells are found in all vascularized organs or tissues in adults and can differentiate into osteoblasts, adipocytes, and chondroblasts.
Hematopoietic Stem Cells (HSCs): Located in the bone marrow, HSCs give rise to all types of blood cells and are integral to bone marrow transplantation procedures.
Neural Stem Cells (NSCs): Found in specific brain areas, NSCs generate the brain's major cell types: nerve cells, astrocytes, and oligodendrocytes.
Epithelial Stem Cells: Present in the lining of the digestive tract and skin, these cells continually regenerate these tissues throughout a person's life.
Skeletal Muscle Stem Cells (Satellite Cells): Responsible for the repair and regeneration of skeletal muscle following injury.
Adipose-Derived Stem Cells (ADSCs): Located in fat tissue, ADSCs can differentiate into various cell types, including adipocytes, cartilage cells, and bone cells.
Nucleus Pulposus Cells: Found in the spinal disc, these cells can differentiate into disc tissue cells.
Salivary Gland Stem Cells: These cells hold potential for treating salivary gland dysfunction.
Each adult stem cell type offers unique characteristics and potential therapeutic applications, with ongoing research needed to fully explore their capabilities and limitations.
Mesenchymal Stem Cells (MSCs)
Standards to define human Mesenchymal Stem Cells (hMSCs), a subset of adult stem cells, for laboratory and preclinical studies include:
Adherence to plastic in standard culture conditions.
Ability to differentiate in vitro into osteoblasts, adipocytes, and chondroblasts.
Specific surface antigen expression, with ≥95% of the cells expressing antigens recognized by CD105, CD73, and CD90. These cells should lack (≤2% positive) antigens CD45, CD34, CD14 or CD11b, CD79a or CD19, and HLA-DR.
Adult Stem Cell Research
Adult stem cell research is a dynamic and rapidly growing field, concentrating on unraveling the unique properties of adult stem cells and their potential for therapeutic applications.
The increasing prevalence of this research is driven by the remarkable abilities of adult stem cells, such as their capacity to differentiate into multiple cell types and the possibility to treat a wide range of diseases, previously untreatable with conventional pharmacological agents.
Key Research Focus Areas
In the realm of adult stem cell research, several critical areas are being explored:
Understanding the Mechanisms of Stem Cell Repair: Researchers are delving into the pathways and mechanisms required for adult stem cell repair. This includes studying the mobilization and homing of stem cells to sites of injury, their immunomodulatory effects, and their role in enhancing vascularization in injured tissues.
Developing Robust Culture Systems: Establishing reliable culture systems is vital for expanding these stem cells in vitro. Such advancements are necessary for significant breakthroughs in stem cell research and for the development of personalized regenerative medicine based on autologous transplantation.
Exploring New Sources of Adult Stem Cells: Ongoing research aims to identify and understand new sources of adult stem cells and their potential therapeutic applications. This includes studying cells from oral mucosal epithelium, dental pulp, and hair follicle bulges for their ability to correct specific deficiencies like limbal stem cell deficiency.
Examining State Policy Impact: The research also extends to examining how state stem cell policies might influence the progress and direction of stem cell research, with permissive policies potentially fostering more robust state performances in pluripotent stem cell research.
Studying Pathways in Stem Cell Migration: A crucial aspect is understanding the biological machinery that determines the patterns of migration and translocation of tissue progenitors, which is of great importance for emerging cell-based therapies and regenerative medicine methodologies.
Looking Forward
Adult stem cell research, with its vast potential for regenerative medicine and disease treatment, is at the forefront of medical science. The field requires continued research to fully grasp the capabilities and limitations of these cells.
As our understanding deepens, adult stem cell research is expected to unlock new avenues in medical treatments, revolutionizing our approach to various health conditions and therapies.
Adult stem cells represent a cornerstone in the realm of regenerative medicine, offering transformative potential for treating various diseases and repairing tissues.
This article explores the diverse types and applications of stem cells, from their initial use in bone marrow transplants to the latest advancements in pluripotent and adult stem cell research.
What are Adult Stem Cells?
Adult stem cells, also known as somatic stem cells, are undifferentiated cells that exist throughout the body after development. Adult stem cells are found in all vascularized organs or tissues in adults.
The key function of adult stem cells is their ability to self-renew and differentiate into at least one specialized cell type (adult stem cell repair), allowing them to replace cells lost through normal wear and tear, injury, or disease.
Stem Cell Repair Mechanisms:
Adult stem cells contribute significantly to the maintenance and repair of body tissues.
They replace cells lost through normal processes, injury, or disease, thanks to their self-renewal and differentiation abilities.
Essential for organ homeostasis.
Critical in responding effectively to injuries.
Their migration to injury sites is a key aspect of their functionality.
Adult stem cells are now known to exist in various tissues, including the umbilical cord blood, bone marrow, adipose tissue, placenta, amniotic fluid, and menstrual blood.
Figure 1 - Adult Stem Cells Dyed
Where are Adult Stem Cells Found?
Adult stem cells can be found from various sources including:
Bone marrow: A rich source of Mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs). MSCs can differentiate into diverse cell types, while HSCs give rise to all blood cell types.
Adipose tissue: Adipose-derived stem cells (ADSCs) found in fat tissue are capable of differentiating into cell types like adipocytes, cartilage cells, and bone cells.
Umbilical cord blood: Contains MSCs, which are used in various therapeutic applications.
Placenta and amniotic fluid: These tissues are sources of MSCs used in regenerative medicine and tissue engineering.
Menstrual blood: Another source of MSCs, with potential therapeutic applications.
Nervous system: Neural stem cells (NSCs) located in specific brain areas can generate nerve cells, astrocytes, and oligodendrocytes.
Nucleus pulposus: These cells, found in spinal discs, have the potential to differentiate into disc tissue cells.
Salivary glands: Salivary gland stem cells are being explored for treating salivary gland dysfunction.
These diverse sources underscore the versatility of adult stem cells in medical research and therapy.
Types of Adult Stem Cells
Adult stem cells can be classified into several types based on their origin and differentiation potential:
Mesenchymal Stem Cells (MSCs): Initially isolated from bone marrow, these cells are found in all vascularized organs or tissues in adults and can differentiate into osteoblasts, adipocytes, and chondroblasts.
Hematopoietic Stem Cells (HSCs): Located in the bone marrow, HSCs give rise to all types of blood cells and are integral to bone marrow transplantation procedures.
Neural Stem Cells (NSCs): Found in specific brain areas, NSCs generate the brain's major cell types: nerve cells, astrocytes, and oligodendrocytes.
Epithelial Stem Cells: Present in the lining of the digestive tract and skin, these cells continually regenerate these tissues throughout a person's life.
Skeletal Muscle Stem Cells (Satellite Cells): Responsible for the repair and regeneration of skeletal muscle following injury.
Adipose-Derived Stem Cells (ADSCs): Located in fat tissue, ADSCs can differentiate into various cell types, including adipocytes, cartilage cells, and bone cells.
Nucleus Pulposus Cells: Found in the spinal disc, these cells can differentiate into disc tissue cells.
Salivary Gland Stem Cells: These cells hold potential for treating salivary gland dysfunction.
Each adult stem cell type offers unique characteristics and potential therapeutic applications, with ongoing research needed to fully explore their capabilities and limitations.
Mesenchymal Stem Cells (MSCs)
Standards to define human Mesenchymal Stem Cells (hMSCs), a subset of adult stem cells, for laboratory and preclinical studies include:
Adherence to plastic in standard culture conditions.
Ability to differentiate in vitro into osteoblasts, adipocytes, and chondroblasts.
Specific surface antigen expression, with ≥95% of the cells expressing antigens recognized by CD105, CD73, and CD90. These cells should lack (≤2% positive) antigens CD45, CD34, CD14 or CD11b, CD79a or CD19, and HLA-DR.
Adult Stem Cell Research
Adult stem cell research is a dynamic and rapidly growing field, concentrating on unraveling the unique properties of adult stem cells and their potential for therapeutic applications.
The increasing prevalence of this research is driven by the remarkable abilities of adult stem cells, such as their capacity to differentiate into multiple cell types and the possibility to treat a wide range of diseases, previously untreatable with conventional pharmacological agents.
Key Research Focus Areas
In the realm of adult stem cell research, several critical areas are being explored:
Understanding the Mechanisms of Stem Cell Repair: Researchers are delving into the pathways and mechanisms required for adult stem cell repair. This includes studying the mobilization and homing of stem cells to sites of injury, their immunomodulatory effects, and their role in enhancing vascularization in injured tissues.
Developing Robust Culture Systems: Establishing reliable culture systems is vital for expanding these stem cells in vitro. Such advancements are necessary for significant breakthroughs in stem cell research and for the development of personalized regenerative medicine based on autologous transplantation.
Exploring New Sources of Adult Stem Cells: Ongoing research aims to identify and understand new sources of adult stem cells and their potential therapeutic applications. This includes studying cells from oral mucosal epithelium, dental pulp, and hair follicle bulges for their ability to correct specific deficiencies like limbal stem cell deficiency.
Examining State Policy Impact: The research also extends to examining how state stem cell policies might influence the progress and direction of stem cell research, with permissive policies potentially fostering more robust state performances in pluripotent stem cell research.
Studying Pathways in Stem Cell Migration: A crucial aspect is understanding the biological machinery that determines the patterns of migration and translocation of tissue progenitors, which is of great importance for emerging cell-based therapies and regenerative medicine methodologies.
Looking Forward
Adult stem cell research, with its vast potential for regenerative medicine and disease treatment, is at the forefront of medical science. The field requires continued research to fully grasp the capabilities and limitations of these cells.
As our understanding deepens, adult stem cell research is expected to unlock new avenues in medical treatments, revolutionizing our approach to various health conditions and therapies.
Role of Daughter Cells
The daughter cells play a significant role in maintaining tissue homeostasis. They replace lost or damaged cells and have the potential to regenerate diseased tissue, thus offering therapeutic potential for many human diseases.
Biological Functions and Benefits of Adult Stem Cells
Adult stem cells play a crucial role in our body's health by maintaining a delicate balance in our tissues. They ensure that our tissues have a constant supply of healthy cells to replace those that are lost or damaged.
Adult stem cells can also form many different cell types in the body, opening up the potential for treating diseases that currently have no cure (source).
Adult Stem Cells and The Immune Response
Adult stem cells have been found to interact with the immune system in a variety of ways. For example, mesenchymal stem cells (MSCs), a type of adult stem cell found in multiple tissues, including bone marrow and fat, have been shown to modulate the immune response.
They can alter the function of immune cells and reduce inflammation, making them potential candidates for treating inflammatory diseases.
References
(1) Zakrzewski W, Dobrzyński M, Szymonowicz M, Rybak Z. Stem cells: past, present, and future. Stem Cell Res Ther. 2019 Feb 26;10(1):68. doi: 10.1186/s13287-019-1165-5. PMID: 30808416; PMCID: PMC6390367.
(2) National Research Council (US) and Institute of Medicine (US) Committee on the Biological and Biomedical Applications of Stem Cell Research. Stem Cells and the Future of Regenerative Medicine. Washington (DC): National Academies Press (US); 2002. CHAPTER TWO, Adult Stem Cells.
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