by University of California - Santa Cruz

Hengen's artistic interpretation of the varied brain wave patterns that produce the fundamental states of sleep and wake. Credit: Keith Hengen

Sleep and wake: They're totally distinct states of being that define the boundaries of our daily lives. For years, scientists have measured the difference between these instinctual brain processes by observing brain waves, with sleep characteristically defined by slow, long-lasting waves measured in tenths of seconds that travel across the whole organ.

For the first time, scientists have found that sleep can be detected by patterns of neuronal activity just milliseconds long, 1,000 times shorter than a second, revealing a new way to study and understand the basic brain wave patterns that govern consciousness. They also show that small regions of the brain can momentarily "flicker" awake while the rest of the brain remains asleep, and vice versa from wake to sleep.

These findings, described in a new study published in the journal Nature Neuroscience, are from a collaboration between the laboratories of Assistant Professor of Biology Keith Hengen at Washington University in St. Louis and Distinguished Professor of Biomolecular Engineering David Haussler at UC Santa Cruz. The research was carried out by Ph.D. students David Parks (UCSC) and Aidan Schneider (WashU).

Over four years of work, Parks and Schneider trained a neural network to study the patterns within massive amounts of brain wave data, uncovering patterns that occur at extremely high frequencies that have never been described before and challenge foundational, long-held conceptions of the neurological basis of sleep and wake.

"With powerful tools and new computational methods, there's so much to be gained by challenging our most basic assumptions and revisiting the question of 'what is a state?'" Hengen said. "Sleep or wake is the single greatest determinant of your behavior, and then everything else falls out from there. So if we don't understand what sleep and wake actually are, it seems like we've missed the boat."

"It was surprising to us as scientists to find that different parts of our brains actually take little naps when the rest of the brain is awake, although many people may have already suspected this in their spouse, so perhaps a lack of male-female bias is what is surprising," Haussler quipped.

Understanding sleep

Neuroscientists study the brain via recordings of the electrical signals of brain activity, known as electrophysiology data, observing voltage waves as they crest and fall at different paces. Mixed into these waves are the spike patterns of individual neurons.

The researchers worked with data from mice at the Hengen Lab in St. Louis. The freely-behaving animals were equipped with a very lightweight headset that recorded brain activity from 10 different brain regions for months at a time, tracking voltage from small groups of neurons with microsecond precision.

This much input created petabytes—which are one million times larger than a gigabyte—of data. David Parks led the effort to feed this raw data into an artificial neural network, which can find highly complex patterns, to differentiate sleep and wake data and find patterns that human observation may have missed. A collaboration with the shared academic computer infrastructure located at UC San Diego enabled the team to work with this much data, which was on the scale of what large companies like Google or Facebook might use.

Knowing that sleep is traditionally defined by slow-moving waves, Parks began to feed smaller and smaller chunks of data into the neural network and asked it to predict whether the brain was asleep or awake.

The team found that the model could differentiate between sleep and wake from just milliseconds of brain activity data. This was shocking to the research team—it showed that the model couldn't have been relying on the slow-moving waves to learn the difference between sleep and wake. Just as listening to a thousandth of a second of a song couldn't tell you if it had a slow rhythm, it would be impossible for the model to learn a rhythm that occurs over several seconds by just looking at random isolated milliseconds of information.

"We're seeing information at a level of detail that's unprecedented," Haussler said. "The previous feeling was that nothing would be found there, that all the relevant information was in the slower frequency waves. This paper says, if you ignore the conventional measurements, and you just look at the details of the high frequency measurement over just a thousandth of a second, there is enough there to tell if the tissue is asleep or not. This tells us that there is something going on a very fast scale—that's a new hint to what might be going on in sleep."

Hengen, for his part, was convinced that Parks and Schneider had missed something, as their results were so contradictory to bedrock concepts drilled into him over many years of neuroscience education. He asked Parks to produce more and more evidence that this phenomenon could be real.

"This challenged me to ask myself, 'To what extent are my beliefs based on evidence, and what evidence would I need to see to overturn those beliefs?'" Hengen said. "It really did feel like a game of cat and mouse, because I'd ask David [Parks] over and over to produce more evidence and prove things to me, and he'd come back and say, 'Check this out.' It was a really interesting process as a scientist to have my students tear down these towers brick by brick, and for me to have to be okay with that."

Local patterns

Because an artificial neural network is fundamentally a black box and does not report back on what it learns from, Parks began stripping away layers of temporal and spatial information to try to understand what patterns the model could be learning from.

Eventually, they got down to the point where they were looking at chunks of brain data just a millisecond long and at the highest frequencies of brain voltage fluctuations.

"We'd taken out all the information that neuroscience has used to understand, define, and analyze sleep for the last century, and we asked, 'Can the model still learn under these conditions?'" Parks said. "This allowed us to look into signals we hadn't understood before."

By looking at this data, they were able to determine that the hyperfast pattern of activity between just a few neurons was the fundamental element of sleep that the model was detecting. Crucially, such patterns cannot be explained by the traditional, slow and widespread waves. The researchers hypothesize that the slow-moving waves may be acting to coordinate the fast, local patterns of activity, but ultimately reached the conclusion that the fast patterns are much closer to the true essence of sleep.

If the slow-moving waves traditionally used to define sleep are compared to thousands of people in a baseball stadium doing the wave, then these fast-moving patterns are the conversations between just a few people deciding to participate in the wave. Those conversations occurring are essential for the overall larger wave to take place, and are more directly related to the mood of the stadium—the wave is a secondary result of that.

Observing flickers

In further studying the hyperlocal patterns of activity, the researchers began to notice another surprising phenomenon.

As they observed the model predicting sleep or wake, they noticed what looked at first like errors, in which for a split second the model would detect wake in one region of the brain while the rest of the brain remained asleep. They saw the same thing in wake states: For a split second, one region would fall asleep while the rest of the regions were awake. They call these instances "flickers."

"We could look at the individual time points when these neurons fired, and it was pretty clear that [the neurons] were transitioning to a different state," Schneider said. "In some cases, these flickers might be constrained to the area of just an individual brain region, maybe even smaller than that."

This compelled the researchers to explore what flickers could mean about the function of sleep, and how they affect behavior during sleep and wake.

"There's a natural hypothesis there; let's say a small part of your brain slips into sleep while you're awake—does that mean your behavior suddenly looks like you're asleep? We started to see that that was often the case," Schneider said.

In observing the behavior of mice, the researchers saw that when a brain region would flicker to sleep while the rest of the brain was awake, the mouse would pause for a second, almost like it had zoned out. A flicker during sleep (one brain region "wakes up") was reflected by an animal twitching in its sleep.

Flickers are particularly surprising because they don't follow established rules dictating the strict cycle of the brain moving sequentially between wake to non-REM sleep to REM sleep.

"We are seeing wake to REM flickers, REM to non-REM flickers—we see all these possible combinations, and they break the rules that you would expect based on a hundred years of literature," Hengen said. "I think they reveal the separation between the macro-state—sleep and wake at the level of the whole animal, and the fundamental unit of state in the brain—the fast and local patterns."

Impact

Gaining a deeper understanding of the patterns that occur at high-frequencies and the flickers between wake and sleep could help researchers better study neurodevelopmental and neurodegenerative diseases, which are both associated with sleep dysregulation. Both Haussler and Hengen's lab groups are interested in understanding this connection further, with Haussler interested in further studying these phenomena in cerebral organoid models, bits of brain tissue grown on a laboratory bench.

"This gives us potentially a very, very sharp scalpel with which to cut into these questions of diseases and disorders," Hengen said. "The more we understand fundamentally about what sleep and wake are, the more we can address pertinent clinical and disease related problems."

On a foundational level, this work helps push forward our understanding of the many layers of complexity of the brain as the organ that dictates behavior, emotion, and much more.

More information: David F. Parks et al, A nonoscillatory, millisecond-scale embedding of brain state provides insight into behavior, Nature Neuroscience (2024). DOI: 10.1038/s41593-024-01715-2

Journal information: Nature Neuroscience 

Provided by University of California - Santa Cruz